Journal: Cancer Gene Therapy
Article Title: Cholenic acid derivative UniPR1331 impairs tumor angiogenesis via blockade of VEGF/VEGFR2 in addition to Eph/ephrin
doi: 10.1038/s41417-021-00379-5
Figure Lengend Snippet: The indicated cells were left unstimulated or stimulated with 10 ng/ml VEGF ( a , b ) or 30 ng/ml FGF2 ( c ) with UniPR1331 (30 μM in a – c or increasing concentrations in b ). Then, cells were analyzed by WB with anti-P-VEGFR2 ( a , b ) or anti-P-FGFR1 ( c ) antibody. Uniform loading was confirmed with anti-VEGFR-2 or anti-tubulin antibody. The results shown are representative of other two that gave similar results. The single lanes have been cropped and reorganized for an easy comparison. b Densitometric quantification of P-VEGFR2 immunoreactive bands normalized to the expression levels of VEGFR2. (mean ± S.E.M. of three independent experiments, * P < 0.005).
Article Snippet: Antibody against focal adhesion kinase (FAK; #sc-558), phosphorylated fibroblast growth factor receptor 1 (FGFR1) (#sc-6458, Y766), and VE-cadherin (#sc-6458) were from Santa Cruz Biotechnology (Santa Cruz, CA).
Techniques: Comparison, Expressing